Researchmap

論文・著作 / Publications

Pages: 1–13

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi url

説明・概要 / Description

Purpose

Microfluidic device design remains time-intensive and heavily reliant on computer-aided design (CAD) expertise, which limits scalability in rapid prototyping, high-throughput experimentation and standardized design workflows. While large language models (LLMs) offer a route toward automated CAD, existing script-based CAD tools, e.g. CADQuery, are prompt-sensitive and prone to geometric inconsistencies. This study aims to enable reliable rapid prototyping by accelerating the design-to-fabrication cycle through an LLM-assisted, Python-mediated framework for microfluidics and performing physical fabrication and dimensional validation to confirm whether designs meet manufacturing tolerances and functional requirements.

Design/methodology/approach

ChatGPT is used to generate a closed-loop Python script, executed through a Python script that generates scalable vector graphics (SVG), drawing exchange format (DXF) and standard tessellation language (STL) outputs of microfluidic geometries, including Spiral Mixers, T-Junctions and Y-Junctions along with script execution time. Each design run is logged with a cryptographic hash to ensure traceability and confirm reproducibility. Physical fabrication using fused deposition modelling and dimensional validation using optical metrology on a digital microscope were essential to confirm whether designs meet manufacturing tolerances and functional requirements. Device functionality was assessed through pressure resistance evaluation, with indicative pressure-drop estimates calculated at leakage onset using the Hagen–Poiseuille approximation.

Findings

ChatGPT/Python workflow substantially reduces iteration time compared with the manual CAD workflow. Post-fabrication measurements showed dimensional deviations up to 12.6% across all geometries while iterative compensation further reduced dimensional deviations. Pressure resistance testing confirmed indicative functional device integrity within low-to-moderate flow regimes, with leakage onset observed at flow rates consistent with fused deposition modelling-related sealing limitations, with no evidence of gross geometric errors in the generated CAD files.

Originality/value

This workflow separates probabilistic LLM interaction from deterministic geometry execution. It provides a traceable route from design generation to exploratory fabrication, supported by cryptographic logging, dimensional validation and iterative compensation.

Light-activated, highly efficient intracellular biomolecular delivery using a titanium nitride micro-array device

2026-07

Nandhini Balasubramaniam, Srabani Kar, Amal Kanti Bera, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: The Analyst

発行者 / Publisher: Royal Society of Chemistry (RSC)

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

説明・概要 / Description

A titanium nitride micro-array device for light-activated cell therapy uses near-infrared pulsed laser irradiation to induce transient membrane pores, thereby enabling the intracellular delivery of biomolecules.

Phototaxis and Motility of Euglena gracilis in Physiological Saline via Stepwise Acclimation for Biohybrid Microrobotics

2026-07-06

Kaiya Endo, Soshi Morimoto, Hayato Obayashi, Takayuki Shibata, Shunya Okamoto, Tuhin Subhra Santra, Moeto Nagai

掲載先 / Source: Micromachines

発行者 / Publisher: MDPI AG

Vol.: 17

No.: 7

Type: scientific_journal

Pages: 815–815

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi url

説明・概要 / Description

Microrobots navigating the human body require biocompatible actuators capable of functioning in physiological fluids. The microalga Euglena gracilis offers precise phototactic control; however, its operational stability in simulated physiological environments remains unproven. Here, we report that a stepwise acclimation process preserves the robotic functionality of E. gracilis in 100% phosphate-buffered saline (PBS), 100% fetal bovine serum (FBS), and a NaCl solution at a concentration equivalent to PBS (137 mM). We compared direct transfer against a graduated adaptation protocol, evaluating morphology, swimming speed, motility rate, and phototaxis. Direct transfer to each medium caused near-total immobilization, whereas stepwise acclimation retained motility. Acclimated cells exhibited size reduction (miniaturization) while maintaining their characteristic eccentricity. These adapted cells sustained a negative phototactic response among the remaining motile population, supporting optical controllability despite reduced swimming speed. These results indicate that stepwise acclimation allows E. gracilis to retain partial motility and phototactic controllability under simulated physiological saline conditions, and that the observed miniaturization and preserved photo-responsiveness may be useful features for future biohybrid microrobotics.

A biomimetic microfluidic triple-compartment periodontium-on-chip model for investigation of inflammatory responses in periodontitis

2026-06

Abinaya R., Kavitha Govarthanan, Lakshmi Krishnan, Ki-Taek Lim, Moeto Nagai, Suresh Rao, Tuhin Subhra Santra

掲載先 / Source: Small

発行者 / Publisher: Wiley

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap

量子技術教育導入を見越したデジタルマイクロミラーデバイスを用いた光波制御システムの構築

2026-06

野田陽太, 永井萌土, 河野託也

掲載先 / Source: 応用物理教育

発行者 / Publisher: 公益社団法人応用物理学会応用物理教育分科会

Vol.: 50

No.: 1

Type: scientific_journal

査読あり / Peer reviewed

researchmap

Fully Automated Multi-Sample Sandwich ELISA Device with Flow-Measurement-based Mold Optimization for Practical Applications

2026-05

Yusuke Kanai, Moeto Nagai, Takayuki Shibata, Yoshiaki Ukita, Shunya Okamoto

掲載先 / Source: Sensors and Actuators B: Chemical

発行者 / Publisher: Elsevier BV

Type: scientific_journal

Pages: 140146–140146

査読あり / Peer reviewed国際誌 / International journal

researchmap doi

Force-Sensor-Integrated Soft Microfinger for Mechanical Stimulation and Reaction Generated Force Evaluation of Zebrafish Larvae

2026-05

Tomohiro Nakatsuka, Hiromi Hirata, Moeto Nagai, Satoshi Konishi

掲載先 / Source: Scientific Reports

発行者 / Publisher: Springer Nature

Type: scientific_journal

査読あり / Peer reviewed招待 / Invited国際誌 / International journal

researchmap

Beyond the Black Box: Resin Viscosity and Tensile Strength as Fabrication Guides for VPP 3D-Printed Microfluidic Molds

2026-04-24

Rifat Hussain Chowdhury, Shunya Okamoto, Takayuki Shibata, Tuhin Subhra Santra, Moeto Nagai

掲載先 / Source: Micro

Vol.: 6

No.: 2

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi url

Reduced Bubbles in a PDMS SlipChip: Magnetic Alignment, Oil-Infused Lubrication, and Geometry Optimization

2026-03-21

Rafia Inaam, Imran M., Hussain Mohammad, Hirofumi Yamamoto, Marcela F. Bolontrade, Shunya Okamoto, Takayuki Shibata, Tuhin Subhra Santra, Moeto Nagai

掲載先 / Source: Applied Sciences

発行者 / Publisher: MDPI AG

Vol.: 16

No.: 6

Type: scientific_journal

Pages: 3040–3040

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi url

説明・概要 / Description

PDMS SlipChips are vital for precision medicine, but their performance often degrades when solutions leak or air pockets become trapped between layers. These failures stem from the inherent stickiness of PDMS and uneven surface contact, as the sliding nature of the device prevents permanent sealing. This work addresses these technical hurdles by integrating magnetic clamping with oil-infused lubrication and refined microwell geometries. A 3D-printed magnetic fixture maintains steady contact pressure during operation, while custom-made microstages provide the precise control needed to align microwells across the x–y plane. By allowing the porous PDMS to absorb silicone oil, we created a stable lubricating interface that prevents leakage and reduces friction without sacrificing mobility. We found that a microwell-to-channel width ratio of five substantially suppresses bubble formation compared with narrower designs. These enhancements ensure the generation of consistent, discrete concentration gradients and establish a reliable platform for high-throughput assays using minute sample volumes.

Advanced Photoporation: Micro‐Nanostructures for Size‐Specific Highly Efficient Biomolecular Delivery

2026-02-07

Ashwini Surendra Shinde, Gayathri R., Nandhini Balasubramaniam, Athira Prasad, Donia Dominic, Moeto Nagai, Srabani Kar, Tuhin Subhra Santra

掲載先 / Source: Small

発行者 / Publisher: Wiley

Vol.: 22

No.: 16

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi url url

説明・概要 / Description

ABSTRACT

With the growing demand for precise and minimally invasive intracellular delivery, photoporation has emerged as a powerful non‐viral strategy. This review presents a comprehensive analysis of photoporation as a versatile intracellular delivery platform, with particular emphasis on the role of micro‐ and nanostructured materials in enabling efficient transport across a wide range of biomolecular sizes. A key novelty of this review is its size‐centric organizational framework, which systematically classifies photoporation strategies based on biomolecular cargo size, from small molecules and nucleic acids to ultralarge assemblies and bacteria, rather than conventional material‐ or laser‐based categorizations. The review examines laser‐induced mechanisms responsible for transient membrane permeabilization and highlights critical material parameters, including composition, size, shape, surface charge, and optical properties, that govern light–matter interactions and delivery efficiency. Comparative evaluation of micro‐ and nanostructured materials across different size regimes provides a practical framework for rational material selection and platform design. In addition, key challenges related to delivery precision, biocompatibility, scalability, and clinical translation are critically discussed alongside emerging optimization strategies. By integrating mechanistic insights with translational considerations, this review provides a structured roadmap for developing safe, efficient, and size‐adaptive photoporation platforms for biological research and therapeutic applications.

Titanium Nitride Nanomaterials—A New Horizon for Light‐activated human Mesenchymal Stem Cell and Cancer Cell Therapy

2026-02-04

Nandhini Balasubramaniam, Ranjith Balakrishnan, Amal Kanti Bera, Moeto Nagai, Rajasekaran Subbarayan, Srabani Kar, Tuhin Subhra Santra

掲載先 / Source: Advanced Therapeutics

発行者 / Publisher: Wiley

Vol.: 9

No.: 2

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi url url

説明・概要 / Description

ABSTRACT

Optoporation enables high‐precision delivery of biomolecules for treating diseases through cell therapy, gene editing, and personalized therapeutics. Nanoparticle‐based optoporation offers high transfection efficiency by minimizing cell damage, thus enhancing the overall cellular health. This study demonstrates the versatility of using Titanium nitride nanoparticles (TiN NPs)‐mediated optoporation for the delivery of small to large cargos, achieving high transfection efficiency alongside cell viability. TiN NPs adsorbed on the cell membrane, when irradiated with a laser fluence of 12.8 mJ/cm 2 at an 850 nm wavelength, generate plasmonic bubbles, facilitating the delivery of cargo into the cells. By using this platform, wide range of cargos/biomolecules such as propidium iodide (PI) dye (668.4 Da), dextran (3 kDa), small interfering RNA (siRNA) (13.3 kDa), enhanced green fluorescence protein (EGFP) expression plasmid DNA (229.4 kDa), and β‐ galactosidase enzyme (465 kDa) are delivered into diverse mammalian cell lines (L929, MG‐63, and N2a) with high transfection efficiency and cell viability. The functionality of the transfected β‐galactosidase enzyme is assessed by measuring its enzymatic activity, verifying the effectiveness of the transfection process. For small PI molecules, MG‐63 cells demonstrated a delivery efficiency of 98% with a cell viability of 99%. In contrast, for oversized cargo (enzyme, 465 kDa), the transfection efficiency and cell viability achieved were 97% and 99%, respectively. Furthermore, human mesenchymal stem cells (hMSCs) are transfected with the EGFP plasmid and β‐galactosidase enzyme, demonstrating a transfection efficiency of 98% and cell viability of 99%. To study the cytotoxicity of TiN NPs, an MTT assay, Kaplan‐Meier survival analysis, and behavioral assessment of the in vivo zebrafish model are conducted. The plots suggest that TiN NPs do not pose neurotoxic effects. Thus, this platform has demonstrated inherent potential for cell reprogramming and applications in medicine, molecular biology, and cellular biology.

Immobilized, micro-patterned graphene nanoflake devices for high-throughput, uniform intracellular biomolecular delivery

2026-01

Donia Dominic, Rajdeep Ojha, Moeto Nagai, Srabani Kar, Tuhin Subhra Santra

掲載先 / Source: Materials Advances

発行者 / Publisher: Royal Society of Chemistry (RSC)

Vol.: 7

No.: 4

Type: scientific_journal

Pages: 2279–2299

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

説明・概要 / Description

Micro-patterned rGO devices enable high-throughput intracellular biomolecular delivery.

Transparent SU-8 Micronozzle Array with Minimal Dead Volume for Parallel Liquid-to-Liquid Sample Ejection

2025-09

Kentaro Tanagi, Anuj Tiwari, Sho Kawaharada, Shunya Okamoto, Takayuki Shibata, Tuhin Subhra Santra, Moeto Nagai

掲載先 / Source: Micro

発行者 / Publisher: MDPI

Vol.: 5

No.: 3

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

A high-speed sequential liquid compartmentalization method for digital loop-mediated isothermal amplification in a microfluidic device

2025-08

Riku Honda, Taketo Saruwatari, Daigo Natsuhara, Yuka Kiba, Shunya Okamoto, Moeto Nagai, Masashi Kitamura, Takayuki Shibata

掲載先 / Source: Lab on a Chip

Vol.: 25

Type: scientific_journal

Pages: 4787–4799

査読あり / Peer reviewed国際誌 / International journal

researchmap doi

Massively parallel photosensitive hydrogel encapsulated single-cell to a cluster of cells patterning and bone regeneration application

2025-08

Sarin Abraham, Gayathri R., Kavitha Govarthanan, Suresh Ranga Rao, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: Lab on a Chip

発行者 / Publisher: Royal Society of Chemistry

Vol.: 25

Type: scientific_journal

Pages: 4735–4755

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

High Throughput Intracellular Delivery Using a 2D Cell‐Squeezing Mechanoporation Device and Its Analysis by a Deep Learning Model

2025-08-21

Pulasta Chakrabarty, Abinaya R., Ryoma Suzuki, Srikanth Vedantam, Suresh Rao, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: Advanced Healthcare Materials

発行者 / Publisher: Wiley

Vol.: 14

No.: 32

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal

researchmap doi url

説明・概要 / Description

Abstract

Cell‐squeezing mechanoporation is a simple method for intracellular delivery. Most studies rely on 1D constrictions; however, the throughput is limited. To enable parallel single‐cell delivery with higher throughput, a 2D cell‐squeezing microfluidic device is designed and fabricated. An array of vertical through‐holes, 8 to 15‐µm in diameter (up to 62 000 holes per device), is fabricated on a 20‐µm thin SU‐8 membrane integrated into a polydimethylsiloxane (PDMS) structure. Intracellular delivery occurred by diffusion as single‐cells are rapidly sheared through the vertical constrictions. The platform demonstrates dextran (4–40‐kDa) delivery into an adherent (HeLa) and a suspension (Jurkat) cell line at throughputs up to 3 million cells min−1. The device also delivers small interfering ribonucleic acid (siRNA) and plasmids into primary human mesenchymal stem cells (hMSCs) and human gingival fibroblasts (hGFs), validating its potential for therapeutic applications. The delivery results are analyzed using “image cytometry”—which combines instance segmentation with a rule‐based image processing system. The automated evaluation of single‐cell states, such as cell diameter and fluorescence intensity, is done, which evaluates cell diameter distribution and fluorescence variation within an experimental sample. Quantification of a mechanoporation‐based intracellular delivery at a single‐cell resolution is performed with an automated deep learning (DL)‐based analysis system.

Combinatorial and Alternating Droplet Printing with Self-regulating Pen-needle-based Micronozzle

2025-07

Muhammad Awais Maqbool, Muhammad Zohaib Maqbool, Shunya Okamoto, Takayuki Shibata, Tuhin Subhra Santra, Moeto Nagai

掲載先 / Source: MRS Advances

発行者 / Publisher: Springer Nature

Vol.: 10

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

Distance-Dependent Spatial Analysis of Micropattern-Generated Shockwave for Cell-Type Specific Intracellular Delivery

2025-06

Aniket Mishra, Shunya Okamoto, Takayuki Shibata, Tuhin Subhra Santra, Sangjin Ryu, Moeto Nagai

掲載先 / Source: Biomedical Microdevices

発行者 / Publisher: Springer Nature

Vol.: 27

No.: 30

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

Parallel dilution microfluidic device for enabling logarithmic concentration generation in molecular diagnostics

2025-06

Akira Miyajima, Fumiya Nishimura, Daigo Natsuhara, Yuka Kiba, Shunya Okamoto, Moeto Nagai, Tadashi Yamamuro, Masashi Kitamura, Takayuki Shibata

掲載先 / Source: Lab on a Chip

Vol.: 25

Type: scientific_journal

Pages: 3242–3253

査読あり / Peer reviewed国際誌 / International journal

researchmap doi

説明・概要 / Description

In this study, we present a genetic diagnostic device with a four-stepwise logarithmic dilution capability for rapid and reliable detection of target nucleic acids in a single operation using the colorimetric loop-mediated isothermal amplification (LAMP) method. An innovative feature is the confluent point with differing microchannel heights ensuring the synchronized inflow of liquids while preventing backflow, even under large volumetric flow rate variations (10-10,000-fold). This enabled the independent generation of each dilution factor under constant pressure.

Light-controlled spatiotemporal manipulation of Euglena gracilis in microfluidic channels

2025-06

Pulasta Chakrabarty, Ryoga Ono, Takuya Kohno, Shunya Okamoto, Takayuki Shibata, Tuhin Subhra Santra, Moeto Nagai

掲載先 / Source: Sensors and Actuators A: Physical

発行者 / Publisher: Elsevier BV

Vol.: 387

Type: scientific_journal

Pages: 116414–116414

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

Development of an autonomous picoliter droplet array generating device with a centrifugal microfluidic system

2025-06-04

Shunya Okamoto, Shota Nakamura, Ayumu Oshita, Moeto Nagai, Takayuki Shibata

掲載先 / Source: Microfluidics and Nanofluidics

発行者 / Publisher: Springer Science and Business Media LLC

Vol.: 29

No.: 7

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal

researchmap doi url url

説明・概要 / Description

In this study, we propose and develop an autonomous picoliter droplet array-generating device using a centrifugal microfluidic system. Droplet arrays play a crucial role in the advancement of chemical analyses, such as digital quantification and digital PCR. In digital quantification, it is important to standardize the size of the droplets and ensure the ease of analysis of the detection reactions within each droplet. Hence, we developed a device that can form droplets of a predetermined size within pre-arranged cup structures simply by flowing liquid and successfully controlled the flow for generating picoliter droplet arrays. The flow control was conducted simply by rotation, and it was not necessary to customize the centrifuge. By optimizing the cup arrangement and these spaces, the device realized that approximately140 pL of highly uniform droplets with an area concentration as high as 32 pieces per square millimeter. Moreover, by implementing an evaporation-prevention function, it was confirmed that the droplets could be retained for more than 30 min. Owing to its simplicity, it is expected to make a significant contribution to the widespread adoption of digital quantification and advancement of analysis.

PDMS SlipChip: Optimizing Sealing, Slipping, and Biocompatibility Using Low-Viscosity Silicone Oils

2025-04

Rafia Inaam, Marcela Fabiana Bolontrade, Shunya Okamoto, Takayuki Shibata, Tuhin Subhra Santra, Moeto Nagai

掲載先 / Source: Micromachines

発行者 / Publisher: MDPI

Vol.: 16

No.: 5

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

Proposal of a Rapid Detection System Using Image Analysis for ELISA with an Autonomous Centrifugal Microfluidic System

2024-11

Shunya Okamoto, Yuto Mori, Shota Nakamura, Yusuke Kanai, Yoshiaki Ukita, Moeto Nagai, Takayuki Shibata

掲載先 / Source: Micromachines

Vol.: 15

No.: 11

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal

researchmap doi

以前の記録 94件を表示 / Show 94 older records

20248件 / items

Infrared Light Activated Highly Efficient Cell Therapy Using an Array of Three-Petaled Titanium Micro-Lily Device

2024-10

Ashwini Shinde, Pallavi Shinde, Moeto Nagai, Srabani Kar, Tuhin Subhra Santra

掲載先 / Source: Advanced Therapeutics

Vol.: 7

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

Self-regulating Pen-needle-based Micronozzle for Printing Array of Nanoliter Droplets under Fluorinated Liquid

2024-08

Muhammad Awais Maqbool, Shunya Okamoto, Takayuki Shibata, Tuhin Subhra Santra, Moeto Nagai

掲載先 / Source: Instrumentation Science & Technology

発行者 / Publisher: Taylor & Francis

Vol.: 53

No.: 3

Type: scientific_journal

Pages: 332–350

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi url

A sequential liquid dispensing method in a centrifugal microfluidic device operating at a constant rotational speed for the multiplexed genetic detection of foodborne pathogens

2024-07

Daigo Natsuhara, Yuka Kiba, Ryogo Saito, Shunya Okamoto, Moeto Nagai, Yusuke Yamauchi, Masashi Kitamura, Takayuki Shibata

掲載先 / Source: RSC Advances

発行者 / Publisher: Royal Society of Chemistry (RSC)

Vol.: 14

No.: 31

Type: scientific_journal

Pages: 22606–22617

査読あり / Peer reviewed

researchmap doi

説明・概要 / Description

We propose a sequential liquid dispensing method in a centrifugal microfluidic device for the multiplexed genetic detection.

Ultra-Low Intensity Light Pulses for Large Cargo Delivery into Hard-to-Transfect Cells using rGO Mixed PDMS Microtip Device

2024-07

Hima Padma, Kavitha Illath, Donia Dominic, Hwan-You Chang, Moeto Nagai, Rajdeep Ojha, Srabani Kar, Tuhin Santra

掲載先 / Source: Lab on a Chip

Vol.: 24

Type: scientific_journal

Pages: 3880–3897

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

Hypoxic Culture of Osteosarcoma Cells in PDMS Microfluidic Chamber and Plastic Bag

2024-05

Rafia Inaam, Marcela Bolontrade, Shunya Okamoto, Takayuki Shibata, Tuhin Subhra Santra, Moeto Nagai

掲載先 / Source: IEEJ Transactions on Sensors and Micromachines

Vol.: 144

No.: 5

Type: scientific_journal

Pages: 94–99

査読あり / Peer reviewed国際共著 / International collaboration

researchmap doi

A microfluidic-based quantitative analysis system for the multiplexed genetic diagnosis of human viral infections using colorimetric loop-mediated isothermal amplification

2024-04

Daigo Natsuhara, Akira Miyajima, Tomoya Bussho, Shunya Okamoto, Moeto Nagai, Masaru Ihira, Takayuki Shibata

掲載先 / Source: Analyst

発行者 / Publisher: Royal Society of Chemistry (RSC)

Vol.: 149

Type: scientific_journal

Pages: 3335–3345

査読あり / Peer reviewed国際誌 / International journal

researchmap doi

説明・概要 / Description

In this study, a microfluidic-based system utilizing colorimetric loop-mediated isothermal amplification (LAMP) is introduced for the quantitative analysis of nucleic acid targets. This system offers a user-friendly and cost-effective platform...

Automatic microdispenser-integrated multiplex enzyme-linked immunosorbent assay device with autonomously driven centrifugal microfluidic system

2024-04

Shunya Okamoto, Moeto Nagai, Takayuki Shibata, Yoshiaki Ukita

掲載先 / Source: RSC Advances

発行者 / Publisher: Royal Society of Chemistry (RSC)

Vol.: 14

No.: 20

Type: scientific_journal

Pages: 13827–13836

査読あり / Peer reviewed

researchmap doi

説明・概要 / Description

A design theory for an autonomously driven dispenser was established. The multiplexed ELISA device was integrated with the dispenser and CLOCK circuits, and successfully prepared a calibration curve of mouse IgG by rotating at a constant speed.

Single-Cell Screening through Cell Encapsulation in Photopolymerized Gelatin Methacryloyl

2024-04

Venkatesh Kumar, Panneer Selvam, Takeru Fukunaga, Yuya Suzuki, Shunya, Okamoto, Takayuki Shibata, Tuhin Subhra Santra, Moeto Nagai

掲載先 / Source: Micro

発行者 / Publisher: MDPI

Vol.: 4

No.: 2

Type: scientific_journal

Pages: 295–304

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

説明・概要 / Description

This study evaluated the potential of gelatin methacryloyl (GelMA) for single-cell screening compared to polyethylene glycol diacrylate (PEGDA). GelMA photopolymerized at 1000–2000 mJ/cm2 produced consistent patterns and supported HeLa cell viability. GelMA (5%w/v) facilitated better cell collection within 2 days due to its shape retention. GelMA demonstrated biocompatibility with HeLa cells exhibiting exponential proliferation and biodegradation over 5 days. The average cell displacement over 2 days was 16 µm. Two targeted cell recovery strategies using trypsin were developed: one for adherent cells encapsulated at 800 mJ/cm2, and another for floating cells encapsulated at 800 mJ/cm2, enabling the selective removal of unwanted cells. These findings suggest GelMA as a promising biomaterial for single-cell screening applications, offering advantages over PEGDA in cell encapsulation and targeted recovery.

202311件 / items

Microfluidic device-based synthesis of highly monodispersed dumbbell-shaped gold nanorods and their biocompatibility evaluation

2023-12

Kavitha Illath, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: Chemical Engineering Science

発行者 / Publisher: Elsevier BV

Vol.: 282

Type: scientific_journal

Pages: 119273–119273

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi url

Microfluidic device-fabricated spiky nano-burflower shape gold nanomaterials facilitate large biomolecule delivery into cells using infrared light pulses

2023-10

Kavitha Illath, Srabani Kar, Ashwini Shinde, Rajdeep Ojha, Dhanya R. Iyer, Nitish R. Mahapatra, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: Lab on a Chip

発行者 / Publisher: Royal Society of Chemistry (RSC)

Vol.: 23

No.: 22

Type: scientific_journal

Pages: 4783–4803

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

説明・概要 / Description

Microfluidics-synthesised spiky burflower gold nanoparticles-sensitised photoporation for highly efficient and uniform intracellular delivery of biomolecules.

2023-05-31

Aniket Mishra, Rafia Inaam, Shunya Okamoto, Takayuki Shibata, Tuhin Subhra Santra, Moeto Nagai

掲載先 / Source: Micromachines

Vol.: 14

No.: 6

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi

Metallic Micro-Ring Device for Highly Efficient Large Cargo Delivery in Mammalian Cells using Infrared Light Pulse

2023-04

Ashwini Shinde, Pallavi Shinde, Srabani Kar, Kavitha Illath, Souvik Dey, Nitish R. Mahapatra, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: Lab on a Chip

Vol.: 23

Type: scientific_journal

Pages: 2175–2192

査読あり / Peer reviewed

researchmap doi

Parallel Photothermal Coalescence of Biocompatible Photocurable PEGDA Droplets

2023-03

Moeto Nagai, Shogo Sato, Shota Hiratsuka, Sho Kawaharada, Shunya Okamoto, Tuhin Subhra Santra, Takayuki Shibata

掲載先 / Source: 電気学会論文誌Ｅ（センサ・マイクロマシン部門誌）

発行者 / Publisher: The Institute of Electrical Engineers of Japan

Vol.: 143

No.: 3

Type: scientific_journal

Pages: 49–54

査読あり / Peer reviewed

researchmap doi cinii_research

説明・概要 / Description

Droplet microfluidics is a powerful tool for high-throughput experimentation, and droplet coalescence is necessary for mixing and chemical reactions. Droplet merging and polymerization will release the limits on particle synthesis and widen the technical potential of droplet microfluidics. Previously, a focused laser beam has been used to induce the coalescence of droplets. This paper reports the parallel photothermal coalescence of biocompatible photocurable polyethylene glycol diacrylate (PEGDA) droplets using a 2D image for developing a more efficient coalescence process. PEGDA droplets with diameters of 18 µm to 50 µm were generated in a microfluidic flow-focusing device and stored in a microchannel. A 2D image of violet light induced the parallel coalescence of PEGDA droplets with diameters of around 30 µm. When continuous-phase oil was replaced with nitrogen, PEGDA droplets were photopolymerized.

Surface Plasmon Resonance Tunable Gold Nanostar Synthesis in a Symmetric Flow-focusing Droplet Device

2023-02

Kavitha Illath, Ashwini Shinde, Priyesh Paremmal, Pallavi Gupta, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: Surfaces and Interfaces

Vol.: 36

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi

Combinatorial physical methods for cellular therapy: Towards the future of cellular analysis?

2023-01

Pulasta Chakrabarty, Kavitha Illath, Srabani Kar, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: Journal of Controlled Release

発行者 / Publisher: Elsevier BV

Vol.: 353

Type: scientific_journal

Pages: 1084–1095

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

Recent Advances of Biosensor-Integrated Organ-on-a-Chip Technologies for Diagnostics and Therapeutics

2023-01-30

Ashwini Shinde, Kavitha Illath, Uvanesh Kasiviswanathan, Shalini Nagabooshanam, Pallavi Gupta, Koyel Dey, Pulasta Chakrabarty, Moeto Nagai, Suresh Rao, Srabani Kar, Tuhin Subhra Santra

掲載先 / Source: Analytical Chemistry

発行者 / Publisher: American Chemical Society (ACS)

Vol.: 95

No.: 6

Type: scientific_journal

Pages: 3121–3146

査読あり / Peer reviewed

researchmap doi url

202210件 / items

Single-cell patterning: a new frontier in bioengineering

2022-12

R. Gayathri, S. Kar, M. Nagai, F.-G. Tseng, P.S. Mahapatra, T.S. Santra

掲載先 / Source: Materials Today Chemistry

発行者 / Publisher: Elsevier BV

Vol.: 26

Type: scientific_journal

Pages: 101021–101021

査読あり / Peer reviewed

researchmap doi

Automated Detection of Patterned Single-Cells within Hydrogel using Deep Learning

2022-10

Tanmay Debnath, Ren Hattori, Shunya Okamoto, Takayuki Shibata, Tuhin Subhra Santra, Moeto Nagai

掲載先 / Source: Scientific Reports

Vol.: 12

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal国際共著 / International collaboration

researchmap doi

Mixing Performance of a Planar Asymmetric Contraction-and-Expansion Micromixer

2022-08

Daigo Natsuhara, Ryogo Saito, Shunya Okamoto, Moeto Nagai, Takayuki Shibata

掲載先 / Source: Micromachines

Vol.: 13

No.: 9

Type: scientific_journal

査読あり / Peer reviewed国際誌 / International journal

researchmap doi pubmed pmc

説明・概要 / Description

Micromixers are one of the critical components in microfluidic devices. They significantly affect the efficiency and sensitivity of microfluidics-based lab-on-a-chip systems. This study introduces an efficient micromixer with a simple geometrical feature that enables easy incorporation in a microchannel network without compromising the original design of microfluidic devices. The study proposes a newly designed planar passive micromixer, termed a planar asymmetric contraction-and-expansion (P-ACE) micromixer, with asymmetric vertical obstacle structures. Numerical simulation and experimental investigation revealed that the optimally designed P-ACE micromixer exhibited a high mixing efficiency of 80% or more within a microchannel length of 10 mm over a wide range of Reynolds numbers (0.13 ≤ Re ≤ 13), eventually attaining approximately 90% mixing efficiency within a 20 mm microchannel length. The highly asymmetric geometric features of the P-ACE micromixers enhance mixing because of their synergistic effects. The flow velocities and directions of the two fluids change differently while alternately crossing the longitudinal centerline of the microchannel, with the obstacle structures asymmetrically arranged on both sidewalls of the rectangular microchannel. This flow behavior increases the interfacial contact area between the two fluids, thus promoting effective mixing in the P-ACE micromixer. Further, the pressure drops in the P-ACE micromixers were experimentally investigated and compared with those in a serpentine micromixer with a perfectly symmetric mixing unit.

A microfluidic diagnostic device with air plug-in valves for the simultaneous genetic detection of various food allergens

2022-07

Daigo Natsuhara, Sae Misawa, Ryogo Saito, Koki Shirai, Shunya Okamoto, Moeto Nagai, Masashi Kitamura, Takayuki Shibata

掲載先 / Source: Scientific Reports

発行者 / Publisher: Springer Science and Business Media LLC

Vol.: 12

No.: 1

Type: scientific_journal

Pages: 12852–12852

査読あり / Peer reviewed国際誌 / International journal

researchmap doi pubmed pmc url url

説明・概要 / Description

Abstract

The identification of accidental allergen contamination in processed foods is crucial for risk management strategies in the food processing industry to effectively prevent food allergy incidents. Here, we propose a newly designed passive stop valve with high pressure resistance performance termed an “air plug-in valve” to further improve microfluidic devices for the detection of target nucleic acids. By implementing the air plug-in valve as a permanent stop valve, a maximal allowable flow rate of 70 µL/min could be achieved for sequential liquid dispensing into an array of 10 microchambers, which is 14 times higher than that achieved with the previous valve arrangement using single-faced stop valves. Additionally, we demonstrate the simultaneous detection of multiple food allergens (wheat, buckwheat, and peanut) based on the colorimetric loop-mediated isothermal amplification assay using our diagnostic device with 10 microchambers compactly arranged in a 20-mm-diameter circle. After running the assays at 60 °C for 60 min, any combination of the three types of food allergens and tea plant, which were used as positive and negative control samples, respectively, yielded correct test results, without any cross-contamination among the microchambers. Thus, our diagnostic device will provide a rapid and easy sample-to-answer platform for ensuring food safety and security.

Effect of Size and Interparticle Distance of Nanoparticles on the Formation of Bubbles Induced by Nanosecond Laser

2022-06

Loganathan MOHAN, Ren HATTORI, Haipeng ZHANG, Yuki MATSUMURA, Tuhin Subhra SANTRA, Takayuki SHIBATA, Sangjin RYU, Moeto NAGAI

掲載先 / Source: Surfaces and Interfaces

Vol.: 30

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi

Standardized Outline of PDMS Microchips with Laser-cut Stacking Mold

2022-03-01

Moeto Nagai, Tuhin Subhra Santra, Takayuki Shibata

掲載先 / Source: 電気学会論文誌Ｅ（センサ・マイクロマシン部門誌）

発行者 / Publisher: Institute of Electrical Engineers of Japan (IEE Japan)

Vol.: 142

No.: 3

Type: scientific_journal

Pages: 43–47

査読あり / Peer reviewed

researchmap doi url

Microfluidic platforms for single neuron analysis

2022-02

Pallavi Gupta, Ashwini Shinde, Kavitha Illath, Srabani Kar, Moeto Nagai, Fan-Gang Tseng, Tuhin Subhra Santra

掲載先 / Source: Materials Today Bio

発行者 / Publisher: Elsevier BV

Vol.: 13

Type: scientific_journal

Pages: 100222–100222

査読あり / Peer reviewed

researchmap doi

Microfluidic Mechanoporation for Cellular Delivery and Analysis

2022-01

Pulasta Chakrabarty, Pallavi Gupta, Kavitha Illath, Srabani Kar, Moeto Nagai, Fan-Gang Tseng, Tuhin Subhra Santra

掲載先 / Source: Materials Today Bio

発行者 / Publisher: Elsevier BV

Vol.: 13

Type: scientific_journal

Pages: 100193–100193

査読あり / Peer reviewed

researchmap doi

Microfluidic Nanomaterials: From Synthesis to Biomedical Applications

2022-01

Kavitha Illath, Srabani Kar, Pallavi Gupta, Ashwini Shinde, Syrpailyne Wankhar, Fan-Gang Tseng, Ki-Taek Lim, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: Biomaterials

Vol.: 280

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi

Revisited Japanese research literature on the stalk contraction and relaxation of stalked ciliates

2022-01

Sangjin RYU, Haipeng ZHANG, Moeto NAGAI

掲載先 / Source: Mechanical Engineering Reviews

発行者 / Publisher: Japan Society of Mechanical Engineers

Vol.: 8

No.: 1

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi url

20217件 / items

Microvalve Actuated by Vorticella: Self-oscillating Valve and Improvement Measures to Calcium-responsive Valve

2021-12

Kohei TANIZAKI, Tuhin Subhra SANTRA, Takayuki SHIBATA, Moeto NAGAI

掲載先 / Source: Mechanical Engineering Journal

Vol.: 8

No.: 6

Type: scientific_journal

査読あり / Peer reviewed国際共著 / International collaboration

researchmap doi

A method of sequential liquid dispensing for the multiplexed genetic diagnosis of viral infections in a microfluidic device

2021-11

Daigo Natsuhara, Ryogo Saito, Hiroka Aonuma, Tatsuya Sakurai, Shunya Okamoto, Moeto Nagai, Hirotaka Kanuka, Takayuki Shibata

掲載先 / Source: Lab on a Chip

発行者 / Publisher: Royal Society of Chemistry (RSC)

Vol.: 21

Type: scientific_journal

Pages: 4779–4790

査読あり / Peer reviewed

researchmap doi

説明・概要 / Description

In this study, we introduce polydimethylsiloxane (PDMS)-based microfluidic devices capable of sequential dispensing of samples into multiple reaction microchambers in a single operation to provide a fast and easy sample-to-answer...

Electrochemical Fabrication of TiO2 Micro-Flowers for an Efficient Intracellular Delivery Using Nanosecond Light Pulse

2021-04

L. Mohan, Srabani Kar, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: Materials Chemistry and Physics

発行者 / Publisher: Elsevier BV

Vol.: 267

Type: scientific_journal

Pages: 124604–124604

査読あり / Peer reviewed

researchmap doi

Fabrication of TiO2 Microspikes for Highly Efficient Intracellular Delivery by Pulse Laser-assisted Photoporation

2021-03

L. Mohan, Srabani Kar, Pallab Sinha Mahapatra, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: RSC Advances

Vol.: 11

Type: scientific_journal

Pages: 9336–9348

査読あり / Peer reviewed国際共著 / International collaboration

researchmap doi

Can Titanium oxide Nanotubes Facilitate Intracellular Delivery by Laser-Assisted Photoporation?

2021-03

L. Mohan, Srabani Kar, Ren-Hattori, Miho Ishii-Teshima, Parthasarathi Bera, Sounak Roy, Tuhin Subhra Santra, Takayuki Shibata, Moeto Nagai

掲載先 / Source: Applied Surface Science

発行者 / Publisher: Elsevier BV

Vol.: 543

Type: scientific_journal

Pages: 148815–148815

査読あり / Peer reviewed

researchmap doi

A Review of Single-Cell Adhesion Force Kinetics and Applications

2021-03-05

Ashwini Shinde, Kavitha Illath, Pallavi Gupta, Pallavi Shinde, Ki-Taek Lim, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: Cells

発行者 / Publisher: {mdpi} {ag}

Vol.: 10

No.: 3

Type: scientific_journal

Pages: 577–577

査読あり / Peer reviewed

researchmap doi

Electrolysis of selectively patterned Vorticella with pneumatic microchambers and electrodes

2021-02

Moeto NAGAI, Ryoga KOHARA, Tuhin Subhra SANTRA, Takayuki SHIBATA

掲載先 / Source: Mechanical Engineering Journal

発行者 / Publisher: Japan Society of Mechanical Engineers

Vol.: 8

No.: 1

Type: scientific_journal

Pages: 20-00254–00254

査読あり / Peer reviewed

researchmap doi url

202012件 / items

Massively parallel intracellular delivery using titanium oxide nanotubes

2020

Loganathan Mohan, Srabani Kar, Ren-Hattori, Miho Ishii-Teshima, Kavitha Illath, Anuj Tiwari, Tuhin Subhra Santra, Takayuki Shibata, Moeto Nagai

掲載先 / Source: MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

発行者 / Publisher: Chemical and Biological Microsystems Society

Type: international_conference_proceedings

Pages: 1256–1257

researchmap

A multiplex genetic diagnostic device integrated with vertical phaseguides capable of autonomous dispensing for the detection of arbovirus infections

2020

Daigo Natsuhara, Kisuke Tanaka, Hiroka Aonuma, Tatsuya Sakurai, Moeto Nagai, Hirotaka Kanuka, Takayuki Shibata

掲載先 / Source: MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

発行者 / Publisher: Chemical and Biological Microsystems Society

Type: international_conference_proceedings

Pages: 1189–1190

researchmap

Fabrication of gold-nanorings for massively parallel intracellular delivery

2020

Loganathan Mohan, Ren-Hattori, Miho Ishii-Teshima, Sathish Sundar Dhilip Kumar, Srabani Kar, Tuhin Subhra Santra, Takayuki Shibata, Moeto Nagai

掲載先 / Source: MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

発行者 / Publisher: Chemical and Biological Microsystems Society

Type: international_conference_proceedings

Pages: 414–415

researchmap

Multi-spot light exposure system for parallel measurement of light-response in algae

2020

Taiki Nakamizu, Masayuki Nagara, Takayuki Shibata, Moeto Nagai

掲載先 / Source: 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

発行者 / Publisher: Chemical and Biological Microsystems Society

Type: international_conference_proceedings

Pages: 764–765

researchmap

Formation of nanostructures on magnesium alloy by anodization for potential biomedical applications

2020-12

Mohan L., Srabani Kar, Nandhini B., Sathish Sundar Dhilip Kumar, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: Materials Today Communications

発行者 / Publisher: Elsevier BV

Vol.: 25

Type: scientific_journal

Pages: 101403–101403

査読あり / Peer reviewed

researchmap doi

Infrared Pulse Laser-Activated Highly Efficient Intracellular Delivery Using Titanium Microdish Device

2020-10-12

Pallavi Shinde, Srabani Kar, Mohan Loganathan, Hwan-You Chang, Fan-Gang Tseng, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: ACS Biomaterials Science & Engineering

発行者 / Publisher: American Chemical Society (ACS)

Vol.: 6

No.: 10

Type: scientific_journal

Pages: 5645–5652

査読あり / Peer reviewed

researchmap doi url

Intracellular Delivery using Anisotropic Gold Nanocrystals Synthesized by Microfluidic Device

2020-09-27

Kavitha Illath, Ashwin Kumar Narasimhan, Pallavi Shinde, Syrpailyne Wankhar, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: 15th IEEE International Conference on Nano/Micro Engineered and Molecular System, NEMS 2020

発行者 / Publisher: Institute of Electrical and Electronics Engineers Inc.

Type: international_conference_proceedings

Pages: 448–452

researchmap doi

A Microfluidic Diagnostic Device Capable of Autonomous Sample Mixing and Dispensing for the Simultaneous Genetic Detection of Multiple Plant Viruses

2020-05-26

Daigo Natsuhara, Keisuke Takishita, Kisuke Tanaka, Azusa Kage, Ryoji Suzuki, Yuko Mizukami, Norikuni Saka, Moeto Nagai, Takayuki Shibata

掲載先 / Source: Micromachines

発行者 / Publisher: MDPI AG

Vol.: 11

No.: 6

Type: scientific_journal

Pages: 540–540

査読あり / Peer reviewed

researchmap doi url

説明・概要 / Description

2020-02-04

Pallavi Shinde, Amogh Kumar, Kavitha, Koyel Dey, L. Mohan, Srabani Kar, Tarun Kumar Barik, Javad Sharifi-Rad, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: Delivery of Drugs: Volume 2: Expectations and Realities of Multifunctional Drug Delivery Systems

発行者 / Publisher: Elsevier Inc.

Type: in_book

Pages: 161–190

researchmap doi

20194件 / items

Nanosecond Laser Irradiation on Cells Using Titanium Thin Film for Massively Parallel Cell Intranuclear Delivery

2019-12-01

Shin Sawai, Harsh Gupta, Tuhin Santra, Azusa Kage, Takayuki Shibata, Moeto Nagai

掲載先 / Source: MHS 2019 - 30th 2019 International Symposium on Micro-NanoMechatronics and Human Science

発行者 / Publisher: Institute of Electrical and Electronics Engineers Inc.

Type: international_conference_proceedings

researchmap doi

Batch Assembly of SU-8 Movable Components in Channel Under Mild Conditions for Dynamic Microsystems:Application to Biohybrid Systems

2019-06

Moeto Nagai, Kohei Tanizaki, Takayuki Shibata

掲載先 / Source: Journal of Microelectromechanical Systems

発行者 / Publisher: Institute of Electrical and Electronics Engineers ({IEEE})

Vol.: 28

No.: 3

Type: scientific_journal

Pages: 419–428

査読あり / Peer reviewed

researchmap doi

Phototactic Algae-Driven Unidirectional Transport of Submillimeter-Sized Cargo in a Microchannel

2019-02-16

Moeto Nagai, Takahiro Hirano, Takayuki Shibata

掲載先 / Source: Micromachines

Vol.: 10

No.: 2

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi

Scalable Hollow Nanoneedle Array Using Stepper Lithography for Parallel Intracellular Delivery

2019-01

Moeto Nagai, Tokuma Miyamoto, Takeshi Hizawa, Takayuki Shibata

掲載先 / Source: Precision Engineering

Vol.: 55

Type: scientific_journal

Pages: 439–446

査読あり / Peer reviewed

researchmap doi

20185件 / items

Uniform Transfection: Shock Wave Generation in Laser Ablation and Microcontact Printing

2018-12-01

Harsh Gupta, Keisuke Funahashi, Naoki Iwasaki, Toshiki Minemura, Shin Sawai, Pallavi Shinde, Tuhin Subhra Santra, Takayuki Shibata, Moeto Nagai

掲載先 / Source: MHS 2018 - 2018 29th International Symposium on Micro-NanoMechatronics and Human Science

発行者 / Publisher: Institute of Electrical and Electronics Engineers Inc.

Type: international_conference_proceedings

researchmap doi

Single-cell Electroporation: Current Trends, Applications and Future Prospects

2018-12-01

Srabani Kar, Mohan Loganathan, Koyel Dey, Pallavi Shinde, Hwan-You Chang, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: Journal of Micromechanics and Microengineering

Vol.: 28

No.: 12

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi web_of_science

Rapid Prototyping of PDMS Microchannels for Animal and Plant Cells Using Cutting Plotter and Double Casting

2018-11

Moeto Nagai, Kyohei Tada, Takayuki Shibata

掲載先 / Source: Mechanical Engineering Letters

Vol.: 4

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi

Current Trends of Microfluidic Single-Cell Technologies

2018-10-12

Pallavi Shinde, Loganathan Mohan, Amogh Kumar, Koyel Dey, Anjali Maddi, Alexander N. Patananan, Fan-Gang Tseng, Hwan-You Chang, Moeto Nagai, Tuhin Subhra Santra

掲載先 / Source: International Journal of Molecular Sciences

Vol.: 19

No.: 10

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi web_of_science

2017-01-01

Takayuki Shibata, Tatsuya Ozawa, Yasuharu Ito, Keita Yamamoto, Moeto Nagai

掲載先 / Source: Japanese Journal of Applied Physics

Vol.: 56

No.: 1

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi web_of_science

20163件 / items

Integration of microorganism Vorticella convallaria and poly (ethylene glycol) diacrylate hydrogel for biohybrid systems

2016-10

Moeto NAGAI, Kei KATO, Takayuki SHIBATA

掲載先 / Source: Mechanical Engineering Letters

Vol.: 2

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi

Underwater motion of hydrogel microstructure by optofluidic lithography studied with gap control and object holding platform

2016-10

Moeto Nagai, Kei Kato, Takayuki Shibata

掲載先 / Source: MICROELECTRONIC ENGINEERING

Vol.: 164

Type: scientific_journal

Pages: 108–114

査読あり / Peer reviewed

researchmap doi web_of_science

Development of Ca2+‐Driven Microvalve Powered by Contractile Fiber of Vorticella Development of Ca2+-Driven Microvalve Powered by Contractile Fiber of Vorticella

2016-01

M Nagai, K Tanizaki, T Kawashima, T Shibata

掲載先 / Source: Electronics and Communications in Japan

Vol.: 99

No.: 1

Type: scientific_journal

Pages: 62–69

査読あり / Peer reviewed

researchmap doi web_of_science

20153件 / items

Characterization of light-controlled Volvox as movable microvalve element assembled in multilayer microfluidic device

2015-06

Moeto Nagai, Michihito Oguri, Takayuki Shibata

掲載先 / Source: JAPANESE JOURNAL OF APPLIED PHYSICS

Vol.: 54

No.: 6

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi web_of_science

Dynamic formation of a microchannel array enabling kinesin-driven microtubule transport between separate compartments on a chip.

2015-05-07

Kazuya Fujimoto, Moeto Nagai, Hirofumi Shintaku, Hidetoshi Kotera, Ryuji Yokokawa

掲載先 / Source: Lab on a chip

Vol.: 15

No.: 9

Type: scientific_journal

Pages: 2055–63

査読あり / Peer reviewed国際誌 / International journal

researchmap doi pubmed

説明・概要 / Description

Microtubules driven by kinesin motors have been utilised as "molecular shuttles" in microfluidic environments with potential applications in autonomous nanoscale manipulations such as capturing, separating, and/or concentrating biomolecules. However, the conventional flow cell-based assay has difficulty in separating bound target molecules from free ones even with buffer flushing because molecular manipulations by molecular shuttles take place on a glass surface and molecular binding occurs stochastically; this makes it difficult to determine whether molecules are carried by molecular shuttles or by diffusion. To address this issue, we developed a microtubule-based transport system between two compartments connected by a single-micrometre-scale channel array that forms dynamically via pneumatic actuation of a polydimethylsiloxane membrane. The device comprises three layers-a control channel layer (top), a microfluidic channel layer (middle), and a channel array layer (bottom)-that enable selective injection of assay solutions into a target compartment and dynamic formation of the microchannel array. The pneumatic channel also serves as a nitrogen supply path to the assay area, which reduces photobleaching of fluorescently labelled microtubules and deactivation of kinesin by oxygen radicals. The channel array suppresses cross-contamination of molecules caused by diffusion or pressure-driven flow between compartments, facilitating unidirectional transport of molecular shuttles from one compartment to another. The method demonstrates, for the first time, efficient and unidirectional microtubule transport by eliminating diffusion of target molecules on a chip and thus may constitute one of the key aspects of motor-driven nanosystems.

Development and characterization of hollow microprobe array as a potential tool for versatile and massively parallel manipulation of single cells

2015-04

Moeto Nagai, Kiyotaka Oohara, Keita Kato, Takahiro Kawashima, Takayuki Shibata

掲載先 / Source: BIOMEDICAL MICRODEVICES

Vol.: 17

No.: 2

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi web_of_science

20143件 / items

Active Mixing in Microchamber using Cilia of Vorticella convallaria

2014-09

Moeto Nagai, Yo Hayasaka, Takahiro Kawashima, Takayuki Shibata

掲載先 / Source: IEEJ TRANSACTIONS ON ELECTRICAL AND ELECTRONIC ENGINEERING

Vol.: 9

No.: 5

Type: scientific_journal

Pages: 575–576

査読あり / Peer reviewed

researchmap doi web_of_science

Reciprocation of micro-objects by contraction and extension of Vorticella convallaria using polylysine as adhesive material

2014-08

Moeto Nagai, Hiroshi Asai, Hiroyuki Fujita

掲載先 / Source: Mechanical Engineering Journal

Vol.: 1

No.: 4

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi

ツリガネムシの収縮性ファイバを利用したCa2+駆動型マイクロバルブの開発 Development of Ca2+-driven microvalve powered by contractile fiber of vorticella

2014-05

永井萌土, 谷_耕平, 川島貴弘, 柴田隆行

掲載先 / Source: 電気学会論文誌 E (センサ・マイクロマシン部門誌)

発行者 / Publisher: Institute of Electrical Engineers of Japan

Vol.: 134

No.: 5

Type: scientific_journal

Pages: 101–107

査読あり / Peer reviewed

researchmap doi

20138件 / items

Mixing of solutions by coordinated ciliary motion in Vorticella convallaria and patterning method for microfluidic applications

2013-11

Moeto Nagai, Yo Hayasaka, Kei Kato, Takahiro Kawashima, Takayuki Shibata

掲載先 / Source: Sensors and Actuators, B: Chemical

Vol.: 188

Type: scientific_journal

Pages: 1255–1262

査読あり / Peer reviewed

researchmap doi

2013-08

Moeto Nagai, Nobuyoshi Matsumoto, Takahiro Kawashima, Takayuki Shibata

掲載先 / Source: MICROELECTRONIC ENGINEERING

Vol.: 108

Type: scientific_journal

Pages: 28–32

査読あり / Peer reviewed

researchmap doi web_of_science

2012

N. Matsumoto, M. Nagai, Y. Hayasaka, M. Oguri, T. Kawashima, T. Shibata

掲載先 / Source: ASIA-PACIFIC INTERDISCIPLINARY RESEARCH CONFERENCE 2011 (AP-IRC 2011)

Vol.: 352

Type: international_conference_proceedings

査読あり / Peer reviewed

researchmap doi web_of_science

ツリガネムシの繊毛運動を用いたマイクロミキサの流れ場解析 Flow field analysis of micromixer powered by ciliary motion of vorticella

2012

早坂陽, 永井萌土, 松本伸賢, 川島貴弘, 柴田隆行

掲載先 / Source: 電気学会E部門誌

発行者 / Publisher: Institute of Electrical Engineers of Japan

Vol.: 132

No.: 3

Type: scientific_journal

Pages: 58–63

査読あり / Peer reviewed

researchmap doi

Development of Cell Culture Microdevice Integrated with Piezoelectric Thin Film Actuator for On-Chip Regulation of Cell Functions

2012-10

Takahiro Kawashima, Yuhei Yamada, Yuuta Matsuzawa, Moeto Nagai, Takayuki Shibata, Toru Masuzawa, Tsuyoshi Kimura, Akio Kishida

掲載先 / Source: JAPANESE JOURNAL OF APPLIED PHYSICS

Vol.: 51

No.: 10

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi web_of_science

Fabrication of hollow SiO2 nanoneedle array and characterization of simultaneous multi-site ion-conductance recordings for cell morphology imaging

2012-10

Takahiro Kawashima, Tomoya Matsugase, Kazumasa Tanaka, Moeto Nagai, Takayuki Shibata, Takashi Mineta, Eiji Makino

掲載先 / Source: MICROELECTRONIC ENGINEERING

Vol.: 98

Type: scientific_journal

Pages: 663–667

査読あり / Peer reviewed

researchmap doi web_of_science

2010

N. Lafitte, M. Kumemura, M. Nagai, L. Jalabert, D. Collard, H. Fujita

掲載先 / Source: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

Vol.: 3

Type: international_conference_proceedings

Pages: 1865–1867

researchmap

Feasibility Study of Cell Culture Microdevice Actuated by Piezoelectric Thin Film for On-chip Regulation of Cell Functions

2010

Takahiro Kawashima, Takayuki Shibata, Moeto Nagai, Toru Masuzawa, Tsuyoshi Kimura, Akio Kishida

掲載先 / Source: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

Vol.: 1

Type: international_conference_proceedings

Pages: 301–303

researchmap

Chemical control of Vorticella bioactuator using microfluidics

2010

Moeto Nagai, Sangjin Ryu, Todd Thorsen, Paul Matsudaira, Hiroyuki Fujita

掲載先 / Source: LAB ON A CHIP

Vol.: 10

No.: 12

Type: scientific_journal

Pages: 1574–1578

査読あり / Peer reviewed

researchmap doi web_of_science

Contraction and extension of Vorticella and its mechanical characterization under flow loading

2010-09

Moeto Nagai, Hiroshi Asai, Hiroyuki Fujita

掲載先 / Source: BIOMICROFLUIDICS

Vol.: 4

No.: 3

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi web_of_science

20092件 / items

Binding of artificial object to vorticella for a microsystem powered by a microorganism

2009-06-06

Nagai Moeto, Kumemura Momoko, Asai Hiroshi, Fujita Hiroyuki

掲載先 / Source: e-Journal of Surface Science and Nanotechnology

Vol.: 7

Type: international_conference_proceedings

Pages: 673–676

査読あり / Peer reviewed

researchmap doi

Three-dimensional two-component velocity measurement of the flow field induced by the Vorticella picta microorganism using a confocal microparticle image velocimetry technique

2009-01

Moeto Nagai, Masamichi Oishi, Marie Oshima, Hiroshi Asai, Hiroyuki Fujita

掲載先 / Source: BIOMICROFLUIDICS

Vol.: 3

No.: 1

Type: scientific_journal

査読あり / Peer reviewed

researchmap doi web_of_science

20071件 / items

Microfluidic Tensile Loading System for Measurement of Mechanical Properties of Vorticella

2007

Moeto Nagai, Momoko Kumemura, Naoyoshi Sakaki, Hiroshi Asai, Hiroyuki Fujita

掲載先 / Source: Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

発行者 / Publisher: Chemical and Biological Microsystems Society

Type: international_conference_proceedings

Pages: 745–747

researchmap

researchmap API 最終取得：2026-11-11 20:21

MISC・その他の文献 / Other Publications97件

最新 25件を表示 / Showing 25 recent records

US-Japan NSF IRES Program for Developing Portable Point-of-Care Testing Devices: Preparation and Experiences of Year 2

2026-03

2024-06

永井萌土

掲載先 / Source: 電気学会誌

Vol.: 144

No.: 6

Type: meeting_report

Pages: 366–366

招待 / Invited

researchmap doi

カラーレジストへのレーザスキャンによる大量オプトポレーション法の開発—Massive optoporation using laser scanning on color resist

2023-11

山本寛文, Aniket Mishra, 馬込壮真, 岡本俊哉, 柴田隆行, 永井萌土

掲載先 / Source: 「センサ・マイクロマシンと応用システム」シンポジウム論文集電気学会センサ・マイクロマシン部門 [編]

発行者 / Publisher: [東京] : Institute of Electrical Engineers of Japan

Vol.: 40

Type: summary_national_conference

2022-03-01

工藤寛之, 永井萌土

掲載先 / Source: 電気学会論文誌Ｅ（センサ・マイクロマシン部門誌）

発行者 / Publisher: Institute of Electrical Engineers of Japan (IEE Japan)

Vol.: 142

No.: 3

Type: introduction_scientific_journal

Pages: 31–31

査読あり / Peer reviewed招待 / Invited

researchmap doi url

カラーレジストからのレーザ誘起衝撃波を用いたオプトポレーション法の開発—Optoporation of cells by laser-induced shock waves from color resist—マグネティックス/マイクロマシン・センサシステム/バイオ・マイクロシステム合同研究会磁気センサ・ライフサイエンス応用センサに関連するセンシング技術の研究発表

2021-12

服部蓮, 松村優基, Loganathan Mohan, Mishra Aniket, 山本寛文, Muhammad Aidee, 手島美帆, 岡本俊哉, 柴田隆行, 永井萌土

掲載先 / Source: 電気学会研究会資料. MAG = The papers of technical meeting on magnetics, IEE Japan / マグネティックス研究会 [編]

発行者 / Publisher: 東京 : 電気学会

Vol.: 2021

No.: 151-168

Type: summary_national_conference

Pages: 55–59

researchmap cinii_research url url

新企画「研究者インタビュー」はじめます New Project "Researcher Interview" Begins!

2021-11-01

萌土永井

掲載先 / Source: 電気学会論文誌 E (センサ・マイクロマシン部門誌)

発行者 / Publisher: Institute of Electrical Engineers of Japan (IEE Japan)

Vol.: 141

No.: 11

Type: introduction_scientific_journal

Pages: NL11_2–NL11_2

査読あり / Peer reviewed

researchmap doi url

国際会議報告: The 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2020)

2020-02-01

永井萌土

掲載先 / Source: 電気学会論文誌 E (センサ・マイクロマシン部門誌)

Vol.: 141

No.: 2

Type: introduction_scientific_journal

researchmap

研究室だより: 豊橋技術科学大学大学院工学研究科機械工学系マイクロ・ナノ機械システム研究室

2019-11-01

以前の記録 72件を表示 / Show 72 older records

20161件 / items

Non-invasive Dielectrophoretic Manipulation of Single Cells Based on Dielectric-coated Array Electrode

2016-01-01

Ryosaku Sakamoto, Keisuke Funahashi, Moeto Nagai, Takayuki Shibata

掲載先 / Source: Ignite 2016

Type: introduction_international_proceedings

査読あり / Peer reviewed

researchmap

201512件 / items

E部門発足20周年記念特集〜未来に向けて（若手研究者の夢）〜：生物と人工物を融合したマイクロシステムの実用化

2015

永井萌土

掲載先 / Source: 電気学会論文誌Ｅ（センサ・マイクロマシン部門誌）

発行者 / Publisher: The Institute of Electrical Engineers of Japan

Vol.: 135

No.: 7

Type: introduction_scientific_journal

Pages: NL7_3–NL7_3

招待 / Invited

researchmap doi url

Micro Fingers for Arranging Single cells?Development of Hollow Microprobe Array for Handling Single Cells in a Parallel Layout?

2015

Moeto Nagai

掲載先 / Source: EurekAlert! Science News, Public Release

Type: introduction_commerce_magazine

2015-03

武藤一夫, 川島貴弘, 永井萌土, 柴田隆行

掲載先 / Source: 八戸工業大学紀要

発行者 / Publisher: 八戸工業大学

Vol.: 34

Pages: 1–6

researchmap

201413件 / items

MEMS-Based Dispenser Array for Selective Immobilization of Molecular Recognition Elements on Bio-Image Sensor

2014

M. Nagai, S. Soga, T. Miyamoto, T. Kawashima, T. Shibata

掲載先 / Source: 2014 International Symposium on Micro-nanomechatronics and Human Science (Mhs)

Type: introduction_international_proceedings

査読あり / Peer reviewed

researchmap web_of_science

Direct Assembly of Movable Parts in a Microchannel Using a Water-Soluble Sacrificial Layer for Biohybrid Systems

2014

M. Nagai, K. Tanizaki, T. Kawashima, T. Shibata

掲載先 / Source: 2014 International Symposium on Micro-nanomechatronics and Human Science (Mhs)

Type: introduction_international_proceedings

査読あり / Peer reviewed

2014-10-20

宮本篤真, 永井萌土, 川島貴弘, 柴田隆行

掲載先 / Source: マイクロ・ナノ工学シンポジウム

発行者 / Publisher: 一般社団法人日本機械学会

Vol.: 2014

No.: 6

Pages: "21pm1–E4-1"-"21pm1-E4-2"

researchmap

説明・概要 / Description

Well-defined tips of nanoneedles are essential for stable and minimally invasive intracellular delivery. We developed fabrication process of needle tips, which are in contact with single cells. An i-line stepper was used to reduce pattern 5.3 times smaller from their reticle sizes. We used circular nano-patterns and fabricated tips of nano-needle array by dry and wet etching of silicon. The diameters of 36 nanoneedles with the minimum openings were 700 nm inner diameter and 1.14 μm outer diameter.

20am2-A6 ツリガネムシと可動構造体の融合による流体抵抗可変型マイクロバルブの開発 20am2-A6 Development of Microvalve with Adjustable Flow Resistance by Integrating Vorticella and Movable Components

2014-10-20

谷崎耕平, 永井萌土, 川島隆弘, 柴田隆行

掲載先 / Source: マイクロ・ナノ工学シンポジウム

発行者 / Publisher: 一般社団法人日本機械学会

Vol.: 2014

No.: 6

Pages: "20am2–A6-1"-"20am2-A6-2"

researchmap

説明・概要 / Description

Biohybrid systems integrating biological motors have been studied for miniature and multifunctional microsystems. The objective of this study is to develop a microvalve composed of Vorticella actuator and a movable component. A movable part was patterned on a water-soluble dextran sacrificial layer and assembled in a microfluidic device. Cells of Vorticella capturing magnetic particles were placed at target position by magnetic force.

J0270203 幹細胞機能解析のための微小環境場の構築 : 集積化プローブおよびマルチウェルによる高効率単一細胞操作([J027-02]細胞および分子のマイクロ・ナノスケール解析(2),バイオエンジニアリング部門) J0270203 Construction of Micro-environment For Function Analysis of Stem Cells : Efficient Manipulation of Single Cells by Combination of Microprobe Array and Microwell Array

2014-09-07

松瀬優也, 永井萌土, 曽我智史, 川島貴弘, 柴田隆行

掲載先 / Source: 年次大会 : Mechanical Engineering Congress, Japan

発行者 / Publisher: 一般社団法人日本機械学会

Vol.: 2014

Pages: "J0270203–1"-"J0270203-5"

researchmap

説明・概要 / Description

Cell manipulation system is an essential tool for reconstruction of cellular microenvironment that leads to high-throughput function analysis. In this paper, we developed a cell manipulator array integrating a cell trapping function on tips, which is a major part of the cell manipulation system. To transport cells electrically, an electrokinetic pump was fabricated and bonded to a PDMS sheet. This pump generated suction and ejection flows through a punched hole. The flow was characterized by measuring trajectory of fluorescent particles. We fabricated electrode pairs for dielectrophoretic cell manipulation. Cells were trapped in between the electrode pairs. We characterized viability of the trapped cells and studied the effect of AC voltages on cells.

超並列シングルセル解析のためのデジタル細胞操作ステーションの開発 (電子デバイス) Development of Digital Cell Manipulation Station for Massively Parallel Analysis of Single Cells

2014-07-10

永井萌土, 松瀬優也, 曽我智史, 河原田翔, 川島貴弘, 柴田隆行

掲載先 / Source: 電子情報通信学会技術研究報告 = IEICE technical report : 信学技報

発行者 / Publisher: 一般社団法人電子情報通信学会

Vol.: 114

No.: 133

Pages: 33–38

researchmap

説明・概要 / Description

生体外で細胞の機能を調べるためには,生体外で細胞を再配列して,生体内の現象を再現することが重要である.本論文では,提案する細胞マニピュレーションシステムのうち,セルフィーダアレイ,マニピュレータアレイについて開発を行った.透明電極であるITOで作製した櫛歯電極にDCバイアスAC信号を印加してポンプとして利用した.これをセルフィーダアレイおよびマニピュレータアレイに活用した.電気浸透流と誘電泳動力を複合して細胞をトラップするセルフィーダアレイを開発した.セルフィーダアレイにて,電圧を印加すると,中央部に向かう電気浸透流が発生した.同時に交流信号を印加させて,正の誘電泳動力により,細胞をウェルアレイ内に配置した.ノズルアレイに電気浸透流ポンプを接続して,マイクロマニピュレータアレイを開発した.電気浸透流ポンプで発生させた電気浸透流により,ノズルアレイへと粒子を吸引した.

307 双方向制御型電気浸透流ポンプを用いた超並列細胞操作技術の開発(OS3-2 MEMSと先進材料(2),OS3 MEMSと先進材料) 307 Massively Parallel Cell Manipulation Technology Using Bidirectional Electroosmotic Pump

2014-03-18

曽我智史, 永井萌土, 加藤啓太, 松瀬優也, 川島貴弘, 柴田隆行

掲載先 / Source: 東海支部総会講演会講演論文集

発行者 / Publisher: 一般社団法人日本機械学会

Vol.: 2014

No.: 63

Pages: "307–1"-"307-2"

researchmap

308 電気化学的プロセスを用いたツリガネムシの透膜処理・運動制御技術の開発(OS3-2 MEMSと先進材料(2),OS3 MEMSと先進材料) 308 Permeabilization and Motion Control of Vorticella by Electrochemical Process

2014-03-18

古原龍河, 永井萌土, 谷崎耕平, 川島貴弘, 柴田隆行

掲載先 / Source: 東海支部総会講演会講演論文集

発行者 / Publisher: 一般社団法人日本機械学会

Vol.: 2014

No.: 63

Pages: "308–1"-"308-2"

researchmap

309 光流体リソグラフィ法を用いたツリガネムシ駆動型マイクロ構造体のその場創成技術(OS3-2 MEMSと先進材料(2),OS3 MEMSと先進材料) 309 In-situ Fabrication of Vorticella-actuated Microstructure by Optofluidic Lithography

2014-03-18

加藤慶, 永井萌土, 川島貴弘, 谷崎耕平, 柴田隆行

掲載先 / Source: 東海支部総会講演会講演論文集

発行者 / Publisher: 一般社団法人日本機械学会

Vol.: 2014

No.: 63

Pages: "309–1"-"309-2"

researchmap

201321件 / items

Development of Cell Culture Microdevice for Regulation of Cell Functions Based on Nanomechanical Stimulation

2013

Genki Umegaki, Yoshitaka Ishihara, Moeto Nagai, Takahiro Kawashima, Takayuki Shibata

掲載先 / Source: 2013 International Symposium on Micro-nanomechatronics and Human Science (Mhs)

Type: introduction_international_proceedings

査読あり / Peer reviewed

researchmap web_of_science

Electrokinetic Intracellular Delivery Combined with Vibration-Assisted Cell Membrane Perforation

2013

Tatsuya Ozawa, Yasuharu Ito, Moeto Nagai, Takahiro Kawashima, Takayuki Shibata

掲載先 / Source: 2013 International Symposium on Micro-nanomechatronics and Human Science (Mhs)

Type: introduction_international_proceedings

査読あり / Peer reviewed

researchmap web_of_science

Fabrication of Movable Microstructure Integrated with Vorticella Actuator

2013

Moeto Nagai, Kei Kato, Takahiro Kawashima, Takayuki Shibata

掲載先 / Source: 2013 International Symposium on Micro-nanomechatronics and Human Science (Mhs)

Type: introduction_international_proceedings

査読あり / Peer reviewed

researchmap web_of_science

微生物と人工物を融合した夢のマイクロシステム

2013

永井萌土

掲載先 / Source: 日本機械学会誌

発行者 / Publisher: 一般社団法人日本機械学会

Vol.: 116

No.: 1140

Type: introduction_scientific_journal

Pages: 793–793

招待 / Invited

researchmap

Particle Transporter Using Cilia of Vorticella

2013

Moeto Nagai, Yo Hayasaka, Takahiro Kawashima, Takayuki Shibata

掲載先 / Source: Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

Type: introduction_international_proceedings

Pages: 67–70

査読あり / Peer reviewed

researchmap doi

Microfluidic Cellular Valve Powerd by Linear Bioactuator

2013

M. Nagai, K. Tanizaki, Y. Hayasaka, T. Kawashima, T. Shibata

掲載先 / Source: 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Type: introduction_international_proceedings

Pages: 1699–1702

査読あり / Peer reviewed

researchmap doi

A Microfluidic Flow-switching Device Powered by Vorticella Stalk

2013

M. Nagai, K. Tanizaki, Y. Hayasaka, T. Kawashima, T. Shibata

掲載先 / Source: Irago Conference 2012

Vol.: 433

Type: introduction_international_proceedings

査読あり / Peer reviewed

researchmap doi web_of_science

ツリガネムシの収縮性ファイバを利用したCa²⁺駆動型マイクロバルブの開発

2013-11-05

谷﨑耕平, 永井萌土, 川島貴弘

掲載先 / Source: 「センサ・マイクロマシンと応用システム」シンポジウム論文集電気学会センサ・マイクロマシン部門 [編]

発行者 / Publisher: Institute of Electrical Engineers of Japan

Vol.: 30

2013-09-08

加藤啓太, 永井萌土, 川島貴弘, 柴田隆行

掲載先 / Source: 年次大会 : Mechanical Engineering Congress, Japan

発行者 / Publisher: 一般社団法人日本機械学会

Vol.: 2013

Pages: "J026034–1"-"J026034-5"

researchmap

説明・概要 / Description

A patterning technique of single cells is required for studying cell communication between multiple cells. The objective of this research is to develop a versatile cell manipulation system capable of patterning of single cells in high precision and super parallel format. Here, we investigate cell manipulation by electroosmotic (EO) flow with a micropipette and microprobe-array. Application of DC voltage (5 V) generated an EO flow through the hollow channels of the pipette and probe-array. Cells stained by fluorescent dye, Calcein-AM were captured at the tip of the pipette and probe-array. Cell damage was characterized through the observation of the cells・ In case of micropipette manipulation, a cell swelled and the fluorescence lost 180 s after the cell capture fi°om optical microscope observation. Cells moved toward some of the microprobe-array by EO flow. The cells were tracked and analyzed. Fluorescence of all the cells disappeared 28 s after the voltage application.

J026035 細胞機能解析のための電場駆動力を利用した細胞内デリバリー技術の開発([J026-03]細胞および分子のマイクロ・ナノスケール解析(3)) J026035 Development of Intracellular Delivery Based on Electrokinetic Forces for Cellular Function Analysis

2013-09-08

小澤辰也, 宮本篤真, 永井萌土, 川島貴弘, 柴田隆行

掲載先 / Source: 年次大会 : Mechanical Engineering Congress, Japan

発行者 / Publisher: 一般社団法人日本機械学会

Vol.: 2013

Pages: "J026035–1"-"J026035-5"

researchmap

説明・概要 / Description

The introduction of biological macromolecules such as DNA, RNA, and proteins into living cells plays a crucial role in the fundamental analysis of cellular functions and mechanisms in living systems. Therefore, we have been developing an effective platform for the in vitro manipulation and analysis of biological cells at the single-cell level based on scanning ion conductance microscopy (SICM) for non-damaging imaging of cells. In this paper, we successfully demonstrated a novel intracellular delivery method of DNA into living HeLa cells via a glass micropipette based on electrokinetically driven flow. We also proposed a vibration-assisted insertion method for penetrating a cell membrane to reduce cell damage. Preliminary insertion tests on a homemade SICM system revealed that the application of the mechanical oscillation can reduce the deformation of cells probably due to an increase in their viscous resistance. Moreover, we also found that a change in the ion current during the insertion process allows us to detect the instant when the micropipette tip penetrates the cell membrane.

超並列デジタル細胞操作ステーションの開発 : 電場駆動力を用いた細胞操作法の基礎的検討 Development of Intelligent Cell Station for Massively Parallel Manipulation of Single Cells : Feasibility Study on Cell Manipulation Based on Electrokinetic Forces

2013-08-09

永井萌土, 松瀬優也, 加藤啓太, 曽我智史, 川島貴弘, 柴田隆行

掲載先 / Source: 電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan

Vol.: 2013

No.: 24

Pages: 11–15

2013-03-08

大原清孝, 永井萌土, 加藤啓太, 川島貴弘, 柴田隆行

掲載先 / Source: 電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan

Vol.: 2013

No.: 1

Pages: 29–33

researchmap

201217件 / items

Electrical Detection of Single-Cell Trapping for Manipulation in an Array-based Format

2012

Moeto Nagai, Keita Kato, Kiyotaka Oohara, Tatsuro Torimoto, Takahiro Kawashima, Takayuki Shibata

掲載先 / Source: 2012 International Symposium on Micro-nanomechatronics and Human Science (Mhs)

Type: introduction_international_proceedings

Pages: 50–53

査読あり / Peer reviewed

researchmap web_of_science

Characteristics of Electrokinetic Flow through Nano Pipette for Cellular Delivery

2012

Moeto Nagai, Tatsuro Torimoto, Tokuma Miyamoto, Takahiro Kawashima, Takayuki Shibata

掲載先 / Source: 2012 International Symposium on Micro-nanomechatronics and Human Science (Mhs)

Type: introduction_international_proceedings

Pages: 194–196

査読あり / Peer reviewed

researchmap web_of_science

2012-10-21

大原清孝, 永井萌土, 加藤啓太, 川島貴弘, 柴田隆行

掲載先 / Source: マイクロ・ナノ工学シンポジウム

発行者 / Publisher: 一般社団法人日本機械学会

Vol.: 2012

No.: 4

Pages: 49–50

researchmap

説明・概要 / Description

We propose fabrication process of micronozzle array with flat tip and establish important part of the process. We demonstrate fabrication of through holes in a Si substrate. Fabricated holes were enlarged by about 3 μm from pattern on mask. Flatness of the tip region was preserved after the etching process. Height of nozzle was controlled by adjusting time of deep silicon etching. Cells were captured and released with a hole-substrate. A cell was captured with a φ5.5 μm nozzle at -50 kPa. With increasing hole diameter, cell internal entry rate increased. We succeeded in release of a cell after cell capture.

OS3-1-4 細胞機能イメージングのための多機能走査型バイオプローブ顕微鏡の開発(OS3 マイクロ・ナノ生体医工学(1)) OS3-1-4 Development of Biological Scanning Probe Microscope for Imaging of Cellular Functions in Living Cells

2012-10-21

中村健志, 小澤辰也, 永井萌土, 川島貴弘, 柴田隆行

掲載先 / Source: マイクロ・ナノ工学シンポジウム

発行者 / Publisher: 一般社団法人日本機械学会

Vol.: 2012

No.: 4

Pages: 47–48

researchmap

説明・概要 / Description

In order to realize spatial and temporal imaging of cellular functions of single living cells, we have been developing a newly designed probe for atomic force microscope (AFM), named "bioprobe", which is integrated with a hollow silicon dioxide (SiO_2) nanoneedle. By employing our newly developed process, bioprobes, which are fully integrated a fluidic microchannel embedded into a silicon (Si) cantilever beam structure, were successfully fabricated. We also proposed a novel intracellular delivery method based on electrokinetically driven flow. Preliminary ejection tests revealed the capability of handling an extremely small volume of liquid with good controllability.

G220014 細胞形態イメージングのための多点同時計測イオンコンダクタンス顕微鏡の開発 G220014 Development of Simultaneous Multi-Site Ion Conductance Microscope for Cell Morphology Imaging

2012-09-09

川島貴弘, 田中一匡, 永井萌土, 柴田隆行, 峯田貴, 牧野英司

掲載先 / Source: 年次大会 : Mechanical Engineering Congress, Japan

発行者 / Publisher: 一般社団法人日本機械学会

Vol.: 2012

Pages: "G220014–1"-"G220014-4"

researchmap

説明・概要 / Description

Scanning ion conductance microscopy (SICM) is a useful tool for imaging of cell morphology in a solution. To further understand cell functions, it is necessary to obtain images of multiple cells. Therefore, we have been developing simultaneous multi-site ion conductance microscope for morphology imaging of multiple cells. This paper reported characterization of multi-site ion conductance recordings by using a modulation method with probe vibration. Furthermore, fabrication of a hollow SiO_2 nanoneedle array using electron beam (EB) lithography, DR1E and standard microfabrication techniques was executed.

G220013 細胞機能イメージングのためのナノニードル搭載型バイオプローブの開発 G220013 Development of Bioprobe Integrated with Nanoneedle for Imaging of Cellular Functions in Living Cells

2012-09-09

中村健志, 小澤辰也, 永井萌土, 川島貴弘, 柴田隆行

掲載先 / Source: 年次大会 : Mechanical Engineering Congress, Japan

発行者 / Publisher: 一般社団法人日本機械学会

Vol.: 2012

Pages: "G220013–1"-"G220013-4"

researchmap

説明・概要 / Description

To introduce novel atomic force microscope (AFM) applications in cellular function analysis of single living cells, we have been developing a newly designed AFM probe, named "bioprobe", which is integrated with a hollow silicon dioxide (SiO_2) nanoneedle. The bioprobe can be used for performing AFM measurements; further, it can also be used to introduce desired biomolecules (DNA, proteins, etc.) into living cells and to extract target biomolecules expressed inside the cells. In this paper, a bioprobe fully integrated a microchannel was successfully fabricated by our newly developed process. Moreover, we demonstrated the ability of the bioprobe to capture AFM images of living cells under near-physiological conditions. Insertion tests also revealed that the nanoneedle can penetrate the cell membrane without significant cell damage. We also proposed a novel intracellular delivery method based on electrokinetically driven flow. The results indicate that the electrokinetic delivery method has the potential to enable the quantitative introduction of biomolecules and the extraction of an extremely small number of biomolecules or cellular components.

細胞マニピュレーションのための単一細胞の電気的検出 Electrical Detection of Single-Cell Trapping for Cell Manipulation

2012-06-12

加藤啓太, 永井萌土, 大原清孝, 鳥本達郎, 川島貴弘, 柴田隆行

掲載先 / Source: 電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan

Vol.: 2012

No.: 8

Pages: 37–40

researchmap

濃度勾配形成デバイスを用いたバイオアクチュエータの動作制御 Motion Control of Bioactuators Using a Gradient Generator

2012-06-12

小栗有寛, 永井萌土, 松本伸賢, 川島貴弘, 柴田隆行

掲載先 / Source: 電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan

Vol.: 2012

No.: 8

Pages: 7–10

researchmap

バイオアクチュエータ融合型フローレギュレータの開発 Development of Flow Regulator Powered by Bioactuators

2012-06-11

永井萌土, 松本伸賢, 小栗有寛, 川島貴弘, 柴田隆行

掲載先 / Source: 電気学会研究会資料. MSS, マイクロマシン・センサシステム研究会

Vol.: 2012

No.: 1

Pages: 43–46

2012-04-01

Rachel E. Pepper, Marcus Roper, Sangjin Ryu, Paul Matsudaira, Nobuyoshi Matsumoto, Moeto Nagai, Howard A. Stone

掲載先 / Source: Integrative and Comparative Biology

Vol.: 52

Type: summary_international_conference

Pages: E135–E135

researchmap web_of_science

20113件 / items

Fabrication of Bioprobe Integrated with Hollow Nanoneedle for Cellular Function Analysis

2011

T. Shibata, N. Kato, S. Horiike, T. Kawashima, M. Nagai, T. Mineta, E. Makino

掲載先 / Source: 2011 Ieee 24th International Conference on Micro Electro Mechanical Systems (Mems)

Type: introduction_international_proceedings

Pages: 861–864

査読あり / Peer reviewed

researchmap web_of_science

Bioactuator:Vorticella Actuation in Microfluidic Systems

2011

Moeto Nagai

掲載先 / Source: Toyohashi University of Technology e-Newsletter

Type: introduction_scientific_journal

招待 / Invited

researchmap

ツリガネムシの運動器官を利用したマイクロ流体素子の開発 Development of Microfluidic Device Using Motor Apparatuses of Vorticella

2011-06-30

松本伸賢, 早坂陽, 永井萌土, 川島貴弘, 柴田隆行

掲載先 / Source: 電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan

Vol.: 2011

No.: 8

Pages: 11–16

researchmap

20102件 / items

マスクレス微細パターン創成技術

2010

柴田隆行, 川島貴弘, 永井萌土

掲載先 / Source: 砥粒加工学会誌

Type: introduction_scientific_journal

招待 / Invited

researchmap

ツリガネムシを駆動源とするマイクロシステム

2010

永井萌土, 浅井博, 藤田博之

掲載先 / Source: 生産研究

発行者 / Publisher: 東京大学生産技術研究所

Vol.: 62

No.: 3

Type: introduction_scientific_journal

Pages: 205–212

researchmap doi

説明・概要 / Description

ツリガネムシを駆動源とするマイクロシステムの設計と実証を行った．本研究は以下の4ステップで行った．1）ツリガネムシの柄と繊毛のアクチュエータの特性理解，2）生体と人工物を融合する手法の確立，3）生体アクチュエータの制御システムの開発，4）繊毛と柄を駆動源するマイクロミキサとマイクロバルブの提案．1）- 3）については実験的により確認し，4）についてはこれらのデバイスの基本構造を提示した．提案したデバイスはマイクロシステムの新領域を開き，制御可能なバイオマイクロシステムへと展開できる．また細胞とMEMSを組み合わせることで，細胞を利用した自律マイクロシステムが将来可能になる．

20091件 / items

マイクロ流体デバイスによるツリガネムシの生体アクチュエータの制御 Control of Bioactuator of Vorticella with a Microfluidic Device

2009-07-23

永井萌土, 藤田博之, RYU Sangjin, THORSEN Todd, MATSUDAIRA Paul

掲載先 / Source: 電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan

Vol.: 2009

No.: 8

Pages: 73–77

researchmap

20081件 / items

ツリガネムシを駆動源とするMEMS作製のためのツリガネムシへの人工物接着 Attachment of Artificial Material onto Vorticella Toward MEMS Powered by Vorticella

2008-06-12

永井萌土, 浅井博, 藤田博之

掲載先 / Source: 電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan

Vol.: 2008

No.: 6

Pages: 31–35

researchmap

20071件 / items

ツリガネムシで駆動するMEMSの実現に向けて : ツリガネムシの力学特性計測とMEMS一体化プロセスの基礎検討 Towards MEMS Powered by Vorticella : Mechanical Measurement of Vorticella and Fundamental Investigation of Integration of Vorticella into MEMS

2007-07-02

永井萌土, 久米村百子, 榊直由, 藤田博之, 浅井博

掲載先 / Source: 電気学会研究会資料. BMS, バイオ・マイクロシステム研究会 = The papers of Technical Meeting on Bio Micro Systems, IEE Japan

Vol.: 2007

No.: 7

Pages: 101–105

researchmap

researchmap API 最終取得：2026-11-11 20:21

書籍・著書 / Books & Chapters12件

最新 12件を表示 / Showing 12 recent records

Metallic nanoparticles for biomedical applications

2021

Kavitha Illath, Syrpailyne Wankhar, Loganathan Mohan, Moeto Nagai, Tuhin Subhra Santra

担当章 / Chapter: Metallic Nanoparticles for Biomedical Applications

出版社 / Publisher: Springer

Pages: 29-81

Total pages: x, 552 p.

Role: joint_work

Type: scholarly_book

査読あり / Peer reviewed

researchmap doi

説明・概要 / Description

Metallic nanoparticles have found various biomedical applications due to their intrinsic physicochemical properties. As the size decreases, the high surface area of particles gives rise to distinctive features, which are entirely different from that of a macro-sized structure. Several methods are involved in synthesizing metallic nanoparticles, and in general, it can be categorized into either bottom-up or top-down approaches. The top-down method consists of cutting down the bulk materials into nano-sized particles through physical, chemical, or mechanical treatments, whereas, in a bottom-up approach, nanoparticles are formed by joining individual atoms or molecules. The top-down approach produces metallic nanoparticles in naked form, which can further agglomerate and hence not suitable for biomedical applications. The bottom-up approach involves solid-state, liquid state, gas phase, biological, microfluidic-technology based, and other methods. Chemical reduction in the bottom-up approach is the most common method of metallic nanoparticle synthesis, which is flexible, simpler, inexpensive, and produces particles in homogenous form. Recently biological method of nanoparticle synthesis has become popular due to its toxic-free nature, inexpensiveness, sustainability, and eco-friendly. In this chapter, we describe the top-down and bottom-up approach and current trends in the synthesis of metallic nanoparticles for biomedical purposes. Further, it explains how the parameters can be tuned to get metallic nanoparticles with the desired shape, size, morphology, composition and crystallinity.

2020-11

Srabani Kar, Pallavi Shinde, Moeto Nagai, Tuhin Subhra Santra

担当章 / Chapter: Chapter 2: Optical Manipulation of Cells

出版社 / Publisher: Jenny Stanford Publishing

Pages: 49-94

Role: joint_work

researchmap doi

Microfluidics and BioMEMS Devices and Applications

2020-11

Kavitha Illath, Ashwin Kumar Narasimahan, Moeto Nagai, Syrpailyne Wankhar, Tuhin Subhra Santra

担当章 / Chapter: Chapter 10 Microfluidics-Based Metallic Nanoparticle Synthesis and Applications

出版社 / Publisher: Jenny Stanford Publishing

Pages: 429-501

Role: joint_work

researchmap doi

Microfluidics and BioMEMS Devices and Applications

2020-11

Hima Manoj, Pallavi Gupta, Loganathan Mohan, Moeto Nagai, Syrpailyne Wankhar, Tuhin Subhra Santra

担当章 / Chapter: Chapter 7: Microneedles: Current Trends and Applications

出版社 / Publisher: Jenny Stanford Publishing

Pages: 275-342

Role: joint_work

researchmap doi

Microfluidics and BioMEMS Devices and Applications

2020-11

Pallavi Gupta, Nandhini Balasubramaniam, Kiran Kaladharan, Fan-Gang Tseng, Moeto Nagai, Hwan-You Chang, Tuhin Subhra Santra

担当章 / Chapter: Chapter 4: Microfluidics in Neuroscience

出版社 / Publisher: Jenny Stanford Publishing

Pages: 149-211

Role: joint_work

researchmap doi

Delivery of Drugs Volume 2: Expectations and Realities of Multifunctional Drug Delivery Systems

2020-02

Pallavi Shinde, Amogh Kumar, Kavitha, Koyel Dey, L. Mohan, Srabani Kar, Tarun Kumar, Barik, Javad Sharifi-Rad, Moeto Nagai, Tuhin Subhra Santra

担当章 / Chapter: Chapter 7 - Physical approaches for drug delivery: an overview

出版社 / Publisher: Elsevier

Pages: 161-190

Role: joint_work

researchmap doi

Micro and Nano Fabrication Technology in Jiwang Yan (ed.)

2018-06

Takayuki Shibata, Junji Sasano, Moeto Nagai

担当章 / Chapter: Catalytic AFM-Based Nanofabrication

出版社 / Publisher: Springer, Singapore

Pages: 857-880

Role: joint_work

researchmap doi

新しい薬剤投与デバイスの開発

2014

柴田隆行, 永井萌土, 川島貴弘

担当章 / Chapter: 1章1節[7] 先端傾斜マイクロニードルの作製と穿刺性能評価

出版社 / Publisher: 技術情報協会，東京，

researchmap

精密加工と微細構造の形成技術

2013

柴田隆行, 永井萌土, 川島貴弘

担当章 / Chapter: マスクレス微細パターン形成技術

出版社 / Publisher: 技術情報協会，東京

Pages: 216-225

researchmap

researchmap API 最終取得：2026-11-11 20:21

研究業績Research Achievements of Moeto Nagai

論文・著作 / Publications

論文 / Journal Articles & Papers119件

Photopatternable Hydrogel for Next-Generation Biomedical and Tissue Engineering Applications

Multi-parallel PDMS Nozzles for Biomaterial Droplet Arrays (Rapid Prototyping with VPP 3D-printed Molds)

LLM-driven rapid prototyping of microfluidic devices: a deterministic Python-mediated framework for design, fabrication, and validation

Light-activated, highly efficient intracellular biomolecular delivery using a titanium nitride micro-array device

Phototaxis and Motility of Euglena gracilis in Physiological Saline via Stepwise Acclimation for Biohybrid Microrobotics

A biomimetic microfluidic triple-compartment periodontium-on-chip model for investigation of inflammatory responses in periodontitis

量子技術教育導入を見越したデジタルマイクロミラーデバイスを用いた光波制御システムの構築

Fully Automated Multi-Sample Sandwich ELISA Device with Flow-Measurement-based Mold Optimization for Practical Applications

Force-Sensor-Integrated Soft Microfinger for Mechanical Stimulation and Reaction Generated Force Evaluation of Zebrafish Larvae

Beyond the Black Box: Resin Viscosity and Tensile Strength as Fabrication Guides for VPP 3D-Printed Microfluidic Molds

Reduced Bubbles in a PDMS SlipChip: Magnetic Alignment, Oil-Infused Lubrication, and Geometry Optimization

Advanced Photoporation: Micro‐Nanostructures for Size‐Specific Highly Efficient Biomolecular Delivery

Titanium Nitride Nanomaterials—A New Horizon for Light‐activated human Mesenchymal Stem Cell and Cancer Cell Therapy

Immobilized, micro-patterned graphene nanoflake devices for high-throughput, uniform intracellular biomolecular delivery

Transparent SU-8 Micronozzle Array with Minimal Dead Volume for Parallel Liquid-to-Liquid Sample Ejection

A high-speed sequential liquid compartmentalization method for digital loop-mediated isothermal amplification in a microfluidic device

Massively parallel photosensitive hydrogel encapsulated single-cell to a cluster of cells patterning and bone regeneration application

High Throughput Intracellular Delivery Using a 2D Cell‐Squeezing Mechanoporation Device and Its Analysis by a Deep Learning Model

Combinatorial and Alternating Droplet Printing with Self-regulating Pen-needle-based Micronozzle

Distance-Dependent Spatial Analysis of Micropattern-Generated Shockwave for Cell-Type Specific Intracellular Delivery

Parallel dilution microfluidic device for enabling logarithmic concentration generation in molecular diagnostics

Light-controlled spatiotemporal manipulation of Euglena gracilis in microfluidic channels

Development of an autonomous picoliter droplet array generating device with a centrifugal microfluidic system

PDMS SlipChip: Optimizing Sealing, Slipping, and Biocompatibility Using Low-Viscosity Silicone Oils

Proposal of a Rapid Detection System Using Image Analysis for ELISA with an Autonomous Centrifugal Microfluidic System

Infrared Light Activated Highly Efficient Cell Therapy Using an Array of Three-Petaled Titanium Micro-Lily Device

Self-regulating Pen-needle-based Micronozzle for Printing Array of Nanoliter Droplets under Fluorinated Liquid

A sequential liquid dispensing method in a centrifugal microfluidic device operating at a constant rotational speed for the multiplexed genetic detection of foodborne pathogens

Ultra-Low Intensity Light Pulses for Large Cargo Delivery into Hard-to-Transfect Cells using rGO Mixed PDMS Microtip Device

Hypoxic Culture of Osteosarcoma Cells in PDMS Microfluidic Chamber and Plastic Bag

A microfluidic-based quantitative analysis system for the multiplexed genetic diagnosis of human viral infections using colorimetric loop-mediated isothermal amplification

Automatic microdispenser-integrated multiplex enzyme-linked immunosorbent assay device with autonomously driven centrifugal microfluidic system

Single-Cell Screening through Cell Encapsulation in Photopolymerized Gelatin Methacryloyl

Microfluidic device-based synthesis of highly monodispersed dumbbell-shaped gold nanorods and their biocompatibility evaluation

Microfluidic device-fabricated spiky nano-burflower shape gold nanomaterials facilitate large biomolecule delivery into cells using infrared light pulses

Image-Based Gel Encapsulation of Suspended Single Cells for Parallel Single-Cell Screening

Ultrathin SU-8 membrane for highly efficient tunable cell patterning and massively parallel large biomolecular delivery

Massively Parallel High‐Throughput Single‐Cell Patterning and Large Biomolecular Delivery in Mammalian Cells Using Light Pulses

Visible Pulsed Laser-Assisted Selective Killing of Cancer Cells with PVP-Capped Plasmonic Gold Nanostars

Metallic Micro-Ring Device for Highly Efficient Large Cargo Delivery in Mammalian Cells using Infrared Light Pulse

Parallel Photothermal Coalescence of Biocompatible Photocurable PEGDA Droplets

Surface Plasmon Resonance Tunable Gold Nanostar Synthesis in a Symmetric Flow-focusing Droplet Device

Combinatorial physical methods for cellular therapy: Towards the future of cellular analysis?

Recent Advances of Biosensor-Integrated Organ-on-a-Chip Technologies for Diagnostics and Therapeutics

Single-cell patterning: a new frontier in bioengineering

Automated Detection of Patterned Single-Cells within Hydrogel using Deep Learning

Mixing Performance of a Planar Asymmetric Contraction-and-Expansion Micromixer

A microfluidic diagnostic device with air plug-in valves for the simultaneous genetic detection of various food allergens

Effect of Size and Interparticle Distance of Nanoparticles on the Formation of Bubbles Induced by Nanosecond Laser

Standardized Outline of PDMS Microchips with Laser-cut Stacking Mold

Microfluidic platforms for single neuron analysis

Microfluidic Mechanoporation for Cellular Delivery and Analysis

Microfluidic Nanomaterials: From Synthesis to Biomedical Applications

Revisited Japanese research literature on the stalk contraction and relaxation of stalked ciliates

Microvalve Actuated by Vorticella: Self-oscillating Valve and Improvement Measures to Calcium-responsive Valve

A method of sequential liquid dispensing for the multiplexed genetic diagnosis of viral infections in a microfluidic device

Electrochemical Fabrication of TiO2 Micro-Flowers for an Efficient Intracellular Delivery Using Nanosecond Light Pulse

Fabrication of TiO2 Microspikes for Highly Efficient Intracellular Delivery by Pulse Laser-assisted Photoporation

Can Titanium oxide Nanotubes Facilitate Intracellular Delivery by Laser-Assisted Photoporation?

A Review of Single-Cell Adhesion Force Kinetics and Applications

Electrolysis of selectively patterned <i>Vorticella</i> with pneumatic microchambers and electrodes

Massively parallel intracellular delivery using titanium oxide nanotubes

A multiplex genetic diagnostic device integrated with vertical phaseguides capable of autonomous dispensing for the detection of arbovirus infections

Fabrication of gold-nanorings for massively parallel intracellular delivery

Multi-spot light exposure system for parallel measurement of light-response in algae

Formation of nanostructures on magnesium alloy by anodization for potential biomedical applications

Infrared Pulse Laser-Activated Highly Efficient Intracellular Delivery Using Titanium Microdish Device

Intracellular Delivery using Anisotropic Gold Nanocrystals Synthesized by Microfluidic Device

A Microfluidic Diagnostic Device Capable of Autonomous Sample Mixing and Dispensing for the Simultaneous Genetic Detection of Multiple Plant Viruses

Characterization of Calcium Ion Release from a Polymer-Coated Electrode with a Plasticized PVC Membrane Containing Calcium Salts, and Its Contraction Examination of Vorticella Convallaria

Photocatalytic Nanofabrication and Intracellular Raman Imaging of Living Cells with Functionalized AFM Probes

Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps

Physical approaches for drug delivery: An overview. An overview.

Nanosecond Laser Irradiation on Cells Using Titanium Thin Film for Massively Parallel Cell Intranuclear Delivery

Batch Assembly of SU-8 Movable Components in Channel Under Mild Conditions for Dynamic Microsystems:Application to Biohybrid Systems

Phototactic Algae-Driven Unidirectional Transport of Submillimeter-Sized Cargo in a Microchannel

研究室だより: 豊橋技術科学大学大学院工学研究科機械工学系マイクロ・ナノ機械システム研究室

「多点ノズルを使って効率的に微量な溶液を輸送できますマイクロ液滴輸送用の流路一体型多点マイクロノズルアレイ」